Due to a lack of good manufacturing process checks, there maybe a variable amount of intact messenger RNA in each LNP , “… but even if we agree to only 1 (modRNA strand), and that each one produces 1000 spike protein (due to the persistence of N1-methyl pseudouridine), we are talking your body having to deal with a minimum 30 trillion pathogenic spike proteins2 in a few months time”
Those are numbers way beyond very severe SARS-COV-2 infections: typically at infection peak between 1 and 100 billion virions
, are present in the body.
This one below shows 1.4 trillion modRNA per shot:
New insights into the structure of Comirnaty Covid-19 vaccine: A theory on soft nanoparticles with mRNA-lipid supercoils stabilized by hydrogen bonds
Despite the worldwide success of mRNA-LNP Covid-19 vaccines, the nanoscale structure of these formulations is still poorly understood. To fill this gap, we used a combination of atomic force microscopy (AFM), dynamic light scattering (DLS), transmission electron microscopy (TEM), cryogenic transmission electron microscopy (cryo-TEM) and the determination of LNP pH gradient to analyze the nanoparticles (NPs) in BNT162b2 (Comirnaty), comparing it with the well characterized pegylated liposomal doxorubicin (Doxil). Comirnaty NPs had similar size to Doxil, however, unlike Doxil liposomes, wherein the stable ammonium and pH gradient enables accumulation of 14C-methylamine in the intraliposomal aqueous phase, Comirnaty LNPs lack such pH gradient in spite of the fact that the pH 4, at which LNPs are prepared, is raised to pH 7.2 after loading of the mRNA. Mechanical manipulation of Comirnaty NPs with AFM revealed soft, compliant structures. The sawtooth-like force transitions seen during cantilever retraction implies that molecular strands, corresponding to mRNA, can be pulled out of NPs, and the process is accompanied by stepwise rupture of mRNA-lipid bonds. Unlike Doxil, cryo-TEM of Comirnaty NPs revealed a granular, solid core enclosed by mono- and bilayers. Negative staining TEM shows 2-5 nm electron-dense spots in the liposom’s interior that are aligned into strings, semicircles, or labyrinth-like networks, which may imply crosslink-stabilized supercoils. The neutral intra-LNP core questions the dominance of ionic interactions holding together this scaffold, raising the alternative possibility of hydrogen bonding between the mRNA and the lipids. Such interaction, described previously for another mRNA/lipid complex, is consistent with the steric structure of ionizable lipid in Comirnaty, ALC-0315, displaying free =O and -OH groups. It is hypothesized that the latter groups can get into steric positions that enable hydrogen bonding with the nitrogenous bases in the mRNA. These newly recognized structural features of mRNA-LNP may be important for the vaccine’s efficacy. ### Competing Interest Statement The authors have declared no competing interest.
As for mRNA LNPs, such as Comirnaty, one must consider that the ~1,414 kDa, 4,284 nucleotide-containing mRNA in Comirnaty has an extended length of ~1,500 nm, which is roughly 180-times longer than a siRNA. The huge size difference between siRNA and mRNA has been well recognized,37
yet this dissimilarity is not illustrated in most schematic cartoons of mRNA-LNP models. For example, in one, the minimally larger mRNA (compared to siRNA) is shown as being bound to the charged inner layer of inverted micelles made of IPC (Fig. 5G
), or as spirals covered by IPC lipids (Fig. 5H, I
). Yet another model of mRNA-LNP shows randomly distributed mRNA in an amorphous lipid core. The envelope of mRNA-LNPs is variably shown as phospholipid enriched monolayer (Fig. 5G, I
If you have not practiced ozone therapy you would not know that ozone goes through several catalytic processes as it gets into the blood stream. Blood is salty helps the process. It's instantly catalyzed into hydrogen peroxide and singlet ionic oxygen which is not a free radical unless the fluid has a PH of 8. We all know that no one living has a PH of 8.
Ozonated water will absorb O3 faster with a pinch of salt. If stored in glass and refrigerated it will keep it's ozone aproximately 16 to 18 hours. In plastic it lasts 8 to 10 hours.
What flows in our veins is not just water. It has many components that make it our living fluid. Without it we die!
Absolutely fascinating article! Thanks 😊
There are several calculations out there, one such calculation for LNP quantities per shot can be found here:
https://www.linkedin.com/mwlite/feed/posts/claverie-jean-michel-9260016_les-vaccins-mrna-sont-ils-surdos%C3%A9s-activity-6872959582122110976-RF6w/
From Marc Giradots Substack post
, those equate to:
50 billion viral vectors for AstraZeneca
40 billion LNPs for Moderna
and likely 10 to 12 billion for Pfizer
Due to a lack of good manufacturing process checks, there maybe a variable amount of intact messenger RNA in each LNP , “… but even if we agree to only 1 (modRNA strand), and that each one produces 1000 spike protein (due to the persistence of N1-methyl pseudouridine), we are talking your body having to deal with a minimum 30 trillion pathogenic spike proteins2 in a few months time”
Those are numbers way beyond very severe SARS-COV-2 infections: typically at infection peak between 1 and 100 billion virions
, are present in the body.
This one below shows 1.4 trillion modRNA per shot:
New insights into the structure of Comirnaty Covid-19 vaccine: A theory on soft nanoparticles with mRNA-lipid supercoils stabilized by hydrogen bonds
Despite the worldwide success of mRNA-LNP Covid-19 vaccines, the nanoscale structure of these formulations is still poorly understood. To fill this gap, we used a combination of atomic force microscopy (AFM), dynamic light scattering (DLS), transmission electron microscopy (TEM), cryogenic transmission electron microscopy (cryo-TEM) and the determination of LNP pH gradient to analyze the nanoparticles (NPs) in BNT162b2 (Comirnaty), comparing it with the well characterized pegylated liposomal doxorubicin (Doxil). Comirnaty NPs had similar size to Doxil, however, unlike Doxil liposomes, wherein the stable ammonium and pH gradient enables accumulation of 14C-methylamine in the intraliposomal aqueous phase, Comirnaty LNPs lack such pH gradient in spite of the fact that the pH 4, at which LNPs are prepared, is raised to pH 7.2 after loading of the mRNA. Mechanical manipulation of Comirnaty NPs with AFM revealed soft, compliant structures. The sawtooth-like force transitions seen during cantilever retraction implies that molecular strands, corresponding to mRNA, can be pulled out of NPs, and the process is accompanied by stepwise rupture of mRNA-lipid bonds. Unlike Doxil, cryo-TEM of Comirnaty NPs revealed a granular, solid core enclosed by mono- and bilayers. Negative staining TEM shows 2-5 nm electron-dense spots in the liposom’s interior that are aligned into strings, semicircles, or labyrinth-like networks, which may imply crosslink-stabilized supercoils. The neutral intra-LNP core questions the dominance of ionic interactions holding together this scaffold, raising the alternative possibility of hydrogen bonding between the mRNA and the lipids. Such interaction, described previously for another mRNA/lipid complex, is consistent with the steric structure of ionizable lipid in Comirnaty, ALC-0315, displaying free =O and -OH groups. It is hypothesized that the latter groups can get into steric positions that enable hydrogen bonding with the nitrogenous bases in the mRNA. These newly recognized structural features of mRNA-LNP may be important for the vaccine’s efficacy. ### Competing Interest Statement The authors have declared no competing interest.
https://www.biorxiv.org/content/10.1101/2022.12.02.518611v1
As for mRNA LNPs, such as Comirnaty, one must consider that the ~1,414 kDa, 4,284 nucleotide-containing mRNA in Comirnaty has an extended length of ~1,500 nm, which is roughly 180-times longer than a siRNA. The huge size difference between siRNA and mRNA has been well recognized,37
yet this dissimilarity is not illustrated in most schematic cartoons of mRNA-LNP models. For example, in one, the minimally larger mRNA (compared to siRNA) is shown as being bound to the charged inner layer of inverted micelles made of IPC (Fig. 5G
), or as spirals covered by IPC lipids (Fig. 5H, I
). Yet another model of mRNA-LNP shows randomly distributed mRNA in an amorphous lipid core. The envelope of mRNA-LNPs is variably shown as phospholipid enriched monolayer (Fig. 5G, I
) or bilayer (Fig. 5H
).
Chalk Tablets and Snake Oil
Wow - comprehending all this info - another wow!
Thank you. Any info on Scalar Energy Healing? thehealinguniverse.com
If you have not practiced ozone therapy you would not know that ozone goes through several catalytic processes as it gets into the blood stream. Blood is salty helps the process. It's instantly catalyzed into hydrogen peroxide and singlet ionic oxygen which is not a free radical unless the fluid has a PH of 8. We all know that no one living has a PH of 8.
Ozonated water will absorb O3 faster with a pinch of salt. If stored in glass and refrigerated it will keep it's ozone aproximately 16 to 18 hours. In plastic it lasts 8 to 10 hours.
What flows in our veins is not just water. It has many components that make it our living fluid. Without it we die!
Thank you